Gc-globulin (Actin-free) ELISA Kit
Cat.No. KIT 034
Numbers of tests:
96 wells
Assay time:
1 hour
Result type:
Quantitative
Regulatory status:
CE IVD
Assess serum Gc-globulin levels to obtain important information about prognosis and progress in critically ill patients suffering from:
- Fulminant liver failure
- Acetaminophen overdose
- Multiple trauma
- Sepsis
Using our Gc-globulin (Actin-free) ELISA Kit you can determine Gc-globulin levels in serum or plasma in less than 1 hour.
The fast procedure and easy-to-use ELISA format makes this kit ideal for prognostic assessment and monitoring progress after appropriate treatment has started.
KIT FEATURES
- The kit is CE IVD-marked
- Results within 1 hour
- Highly reproducible and easy to use
- Ready-to-use calibrators and working solutions
- Pre-coated ELISA strips
- All incubations performed at room temperature
- Measures Gc-globulin in both serum and plasma
- Detection range
- At the recommended sample dilution, the assay range is 5 – 500 µg/L, covering values from normal to critically ill patients
- Easy storage
- All components are stored at 4°C
- Requires only standard ELISA equipment
References:
Background information:
- Harper KD, McLeod JF, Kowalski MA, Haddad JG (1987) Vitamin D binding protein sequesters monomeric actin in the circulation of the rat. J Clin Invest 79:1365-1370.
- Haddad JG, Harper KD, Guoth M, Pietra GG, Sanger JW (1990) Angiopathic consequences of saturating the plasma scavenger system for actin. Proc Natl Acad Sci USA 87:1381-1385.Haddad JG (1995) Plasma vitamin D-binding protein (Gc-globulin): multiple tasks. J Steroid Biochem Molec Biol 53:579 582.
- Yamamoto N (1996) Structural definition of a potent macrophage activating factor derived from vitamin D3-binding protein with adjuvant activity for antibody production. Mol Immunol 33:1157-1164.
- White P, Cooke N (2000) The multifunctional properties and characteristics of vitamin D-binding protein. Tren. Endocrinol Metabol 11:320-327.
Hepatology:
- Schiødt FV, Bondesen S, Tygstrup N (1995) Serial measurements of serum Gc-globulin in acetaminophen intoxication. Eur J Gastroenterol Hepatol 7:635-640.
- Lee WM, Galbraith RM, Watt GH, Hughes RD, McINtire DD, Hoffman BJ, Williams R (1995) Predicting survival in fulminant hepatic failure using serum Gc protein concentrations. Hepatology 21:101-105.
- Schiødt F, Bondesen S, Petersen I, Dalhoff K, Ott P, Tygstrup N (1996) Admission levels of serum Gc-globulin: predictive value in fulminant hepatic failure. Hepatology 23:713-718.
- Schiødt FV, Ott P, Bondesen S, Tygstrup N (1997) Reduced serum Gc-globulin levels in patients with fulminant hepatic failure: association with multiple organ failure. Crit Care Med 25:1366-1370.
- Schiødt FV, Ott P, Tygstrup N, Dahl B, Bondesen S (2001) Temporal profile of total, bound, and free Gc-globulin after acetaminophen overdose. Liver Transpl 7:732-738.
- Schiødt FV, Clemmesen JO, Bondesen S, Dahl, B, Ott P (2001) Increased turnover of Gc-globulin in patients with hepatic encephalopathy. Scand J Gastroenterol 36:998-1003.
Trauma:
- Dahl B. Schiødt FV, Nielsen M, Kiær T, Williams JG, Ott P (1999) Admission levels of Gc-globulin predicts outcome after multiple trauma. Injury 30:275-281.
- Dahl B, Schiødt FV, Ott P, Wians F, Lee WM, Balko J, O'Keefe GE (2003) Plasma concentration of Gc-globulin is associated with organ dysfunction and sepsis after injury. Crit Care Med 31:152-156.
Sepsis:
- Lee WM, Reines D, Watt GH, Cook JA, Wise WC, Halushka PV, Galbraith RM (1989) Alterations in Gc levels and complexing in septic shock. Circ Shock 28:249-255
- Dahl B, Schiødt FV, Ott P, Wians F, Lee WM, Balko J, O'Keefe GE (2003) Plasma concentration of Gc-globulin is associated with organ dysfunction and sepsis after injury. Crit Care Med 31:152-156.

