Anti-Albumin (bovine, BSA)

Mouse monoclonal antibody

Cat.No. HYB 267-01

Subclass IgG1/k + IgG2b/k
Presentation:

Preparation: Protein-A/G purified
Content: Available in 200 μL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.

Antigen:

Bovine serum albumin (BSA) is the most abundant protein in bovine plasma. BSA is produced in the liver, has a molecular mass of 66.4 kDa and its concentration in bovine plasma or serum is 35-50 g/L.

Immunogen:

BSA purified from bovine plasma and adsorbed to aluminum hydroxide gel

Specificity:

HYB 267-01 reacts strongly with BSA, cross-reacts about 25% with human serum albumin but shows no cross-reaction with rabbit serum albumin. Its crossreactivity with albumins from other species has not been tested.

Epitope specificity:

Not determined

Reactivity:

Strong reaction is seen in ELISA with BSA coated directly onto the microtiter well and in Western blotting after SDS-PAGE.

Application:
Method Usability References
ELISA Yes
Immunoblotting Yes
Immunohistochemistry Not determined

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