Anti Serum albumin (human, HSA)
mouse monoclonal antibody
Cat.No. HYB 192-02
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide
Storage: In the dark at 4-8ºC
Human serum albumin (HSA) consists of a single polypeptide chain of 584 aminoacids, stabilized by 17 disulfide bridges. HSA is the most common protein in serum, it is produced in the liver and the concentration in serum is 35-50 mg/ml. HSA is often used as a carrier molecule because of its binding capacity, and thus mainly functions as the regulator of the colloidal osmotic pressure of the blood. The molecular weight is 67.5 kDa (1).
Native HSA isolated from human plasma
HYB 192-02 has specificity for native human serum albumin (HSA)
HYB 192-02 differs from 192-01 and 193-05, but overlap, as determined by inhibition ELISA
HYB 192-02 reacts strongly with native HSA. Strong reaction is seen in ELISA with native HSA directly coated onto the microtiterwell, also when tested in catching ELISA in combination with a polyclonal antibody against HSA (eg. DAKO A 001). In Western blot after SDS-PAGE, HYB 192-02 reacts with native HSA in nonreduced form only.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:8000 | |
| Immunoblotting | Yes | ||
| Immunohistochemistry | Not determined |
1. Scott T & Eagleson M (1988) Concise Encyclopedia Biochemistry: Walter de Gruyter, New York.
