Anti Serum albumin (human, HSA)
Mouse monoclonal antibody
Cat.No. HYB 192-01
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
Human serum albumin (HSA) consists of a single polypeptide chain of 584 aminoacids, stabilized by 17 disulfide bridges. HSA is the most common protein in serum, it is produced in the liver and the concentration in serum is 35-50 mg/ml. HSA is often used as a carrier molecule because of its binding capacity, and thus mainly functions as the regulator of the colloidal osmotic pressure of the blood. The molecular weight is 67.5 kDa (1).
Native HSA isolated from human plasma
HYB 192-01 has specificity for native and denatured human serum albumin (HSA)
HYB 192-01 differs from 192-02 and HYB 193-05, but overlap as determined by inhibition ELISA.
HYB 192-01 reacts strongly with native and denatured HSA. Strong reaction is seen in ELISA with native or denatured HSA directly coated onto the microtiter well, and also in sandwich ELISA in combination with a polyclonal antibody against HSA (e.g. DAKO A 001). In Western blotting after SDS-PAGE, HYB 192-01 reacts with native and denatured HSA in non-reduced form only.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:32,000 | |
| Immunoblotting | Yes | ||
| Immunohistochemistry | Not determined |
1. Scott T & Eagleson M (1988) Concise Encyclopedia Biochemistry: Walter de Gruyter, New York.
