Anti-Haptoglobin

Mouse monoclonal antibody

Cat.No. HYB 170-06

Subclass IgG1/k
Presentation:

Preparation: Protein-A purified
Content: Available in 200 µL and 1 mL volumes, 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15mM sodium azide Storage: In the dark at 4-8ºC

Antigen:

Haptoglobin is an a2-globulin plasma protein synthesized in the liver. It is an acute phase protein which also acts as a scavenger og hemoglobin from hemolyzed red cells. The haptoglobin monomer consists of two a and two b chains; the a chains may be hp1Fa, hp1Sa pr hp2a types while the b chain is constant. Three major phenotypes are recognized: Hp1-1, Hp-2-1 and Hp-2-2, in which the hp1a chain may be either the F or S type

Immunogen:

Haptoglobin purified from pooled human plasma and adsorbed to aluminum hydroxide gel

Specificity:

Reacts with human haptoglobin from pooled plasma. The specificity towards a particular type of a or b chain has not been determined.

Epitope specificity:

Not determined

Reactivity:

Reacts strongly with human haptoglobin from pooled human plasma coated directly onto the ELISA wells. Used as detection antibody in sandwich ELISA with a coat of polyclonal antibody to human haptoglobin.

Application:
Method Usability Dilution guideline References
ELISA Yes 1/32,000
Immunoblotting Not determined
Immunohistochemistry Not determined
Application Remarks:

The dilution guideline for ELISA is based on use as detection antibody for haptoglobin from pooled human plasma coated at 2 µg/mL. Users should determine the optimal dilutions for their own purposes.

 

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