Anti-Haptoglobin
Mouse monoclonal antibody
Cat.No. HYB 170-06
Preparation: Protein-A purified
Content: Available in 200 µL and 1 mL volumes, 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15mM sodium azide Storage: In the dark at 4-8ºC
Haptoglobin is an a2-globulin plasma protein synthesized in the liver. It is an acute phase protein which also acts as a scavenger og hemoglobin from hemolyzed red cells. The haptoglobin monomer consists of two a and two b chains; the a chains may be hp1Fa, hp1Sa pr hp2a types while the b chain is constant. Three major phenotypes are recognized: Hp1-1, Hp-2-1 and Hp-2-2, in which the hp1a chain may be either the F or S type
Haptoglobin purified from pooled human plasma and adsorbed to aluminum hydroxide gel
Reacts with human haptoglobin from pooled plasma. The specificity towards a particular type of a or b chain has not been determined.
Not determined
Reacts strongly with human haptoglobin from pooled human plasma coated directly onto the ELISA wells. Used as detection antibody in sandwich ELISA with a coat of polyclonal antibody to human haptoglobin.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1/32,000 | |
| Immunoblotting | Not determined | ||
| Immunohistochemistry | Not determined |
The dilution guideline for ELISA is based on use as detection antibody for haptoglobin from pooled human plasma coated at 2 µg/mL. Users should determine the optimal dilutions for their own purposes.
