Anti-Haptoglobin (human)

Mouse monoclonal antibody

Cat.No. HYB 170-06

Subclass IgG1/k
Presentation:

Preparation: Protein-A/G purified
Content: Available in 200 μL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.

Antigen:

Haptoglobin is an alpha2-globulin plasma protein synthesized in the liver. It is an acute phase protein which also acts as a scavenger of hemoglobin from hemolyzed red cells. The haptoglobin monomer consists of two alpha and two beta chains; the alpha chains may be hp1Falpha, hp1Salpha or hp2alpha types while the beta chain is constant. Three major phenotypes are recognized: Hp1-1, Hp-2-1 and Hp-2-2, in which the hp1alpha chain may be either the F or S type.

Immunogen:

Haptoglobin purified from pooled human plasma and adsorbed to aluminum hydroxide gel

Specificity:

Reacts with human haptoglobin from pooled plasma. The specificity towards a particular type of a or b chain has not been determined.

Epitope specificity:

Not determined

Reactivity:

Reacts strongly with human haptoglobin from pooled human plasma coated directly onto the ELISA wells. Used as detection antibody in sandwich ELISA with a coat of polyclonal antibody to human haptoglobin.

Application:
Method Usability References
ELISA Yes
Immunoblotting Not determined
Immunohistochemistry Not determined

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