Anti-Gelsolin (human)
Mouse monoclonal antibody
Cat.No. ABS 017-20
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
Gelsolin participates in the extracellular actin scavenger system, binding actin and disaggregating filamentous actin released from dying cells. Actin monomers are then transferred to Gc-globulin. The plasma concentration of gelsolin is reduced in conditions of major actin release, such as acute liver failure, major trauma and rhabdomyolysis, where multi-organ failure is a risk. It has been suggested that gelsolin, like Gc-globulin, can be a prognostic factor in these conditions.
Gelsolin purified from human plasma
ABS 017-20 binds human gelsolin
Not determined
The ELISA dilution guideline applies to gelsolin presented on a coat of polyclonal capture antibody. ABS 017-20 does not work as capture antibody in sandwich ELISA.
| Method | Usability | Dilution guideline | References |
| ELISA | yes | 1:4000 | |
| Immunoblotting | Not determined | ||
| Immunohistochemistry | Not determined |
1. Lee PS, Drager LR, Stossel TP, Moore FD, Rogers SO (2006) Relationship of plasma gelsolin levels to outcomes in critically ill surgical patients. Ann Surg 243:399-403.
