Anti Gc-globulin (human)
mouse monoclonal antibody
Cat.No. HYB 249-05
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide
Storage: In the dark at 4-8ºC
Gc-globulin is a plasma protein produced in the liver. Amongst its ligands are vitamin D, thus Gc-globulin is also called vitamin D-binding protein. Gc-globulin is furthermore part of the actin scavenging system, binding and removing monomeric actin from the blood stream. The molecular mass of Gc-globulin is approximately 50 kDa. The concentration of Gc-globulin in human plasma is app. 400 µg/ml (1).
Gc-globulin isolated from human plasma
HYB 249-05 is specific for human Gc-globulin.
Epitope differs from that of HYB 249-01, HYB 249-02 and HYB 249-10.
HYB 249-05 reacts strongly with Gc-globulin. Strong reaction is seen when tested in sandwich ELISA in combination with a polyclonal antibody against Gc-globulin (e.g. DAKO A0021).
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:25,000 | |
| Immunoblotting | Not determined | ||
| Immunohistochemistry | Not determined |
1. Masuda S, Okano T, Osawa K, Shinjo M, Suematsu T, Kobayashi T (1989) Concentrations of vitamin D-binding protein and vitamin D metabolites in plasma of patients with liver cirrosis. J Nutr Sci Vitaminol 35:225-34.
