Anti Gc-globulin (human), biotinylated
Mouse Monoclonal Antibody
Cat.No. HYB 249-01B
Preparation: Biotinylated
Content: 50 µL, 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15mM sodium azide
Storage: In the dark at 4-8ºC
Gc-globulin is a plasma protein produced in the liver. Amongst its ligands are vitamin D, thus Gc-globulin is also called vitamin D-binding protein. Gc-globulin is furthermore part of the actin scavenging system, binding and removing monomeric actin from the blood stream. The molecular mass of Gc-globulin is approximately 50 kDa. The concentration of Gc-globulin in human plasma is app. 400 µg/ml (1).
Gc-globulin isolated from human plasma (Sigma product)
HYB 249-01 is specific for human Gc-globulin.
Epitope specificity differs from that of HYB 249-02, HYB 249-05 and HYB 249-10.
HYB 249-01 reacts strongly with Gc-globulin. Strong reaction is seen in ELISA with Gc-globulin coated directly onto the microtiter well and when tested in sandwich ELISA in combination with a polyclonal antibody against Gc-globulin (e.g. DAKO A 0021).
In Western blotting after SDS-PAGE, HYB 249-01 reacts with Gc-globulin in both reduced as well as unreduced forms.
| Method | Usability | Dilution guideline | References |
| ELISA | |||
| Immunoblotting | |||
| Immunohistochemistry |
1. Masuda S, Okano T, Osawa K, Shinjo M, Suematsu T, Kobayashi T (1989) Concentrations of vitamin D-binding protein and vitamin D metabolites in plasma of patients with liver cirrosis. J Nutr Sci Vitaminol 35:225-34.
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