Anti-Biotin
Mouse monoclonal antibody
Cat.No. HYB 212-01
Preparation: Protein-A/G purified
Content: Available in 200 μL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.
Biotin is a natural occurring vitamin also called vitamin B4. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da (1).
Biotinylated denatured ovalbumin adsorbed onto aluminum hydroxide gel
HYB 212-01 is specific for biotin
Not determined
HYB 212-01 reacts strongly with biotin. A strong reaction is seen in ELISA with biotin/OA directly coated onto the microtiter well, also when tested in sandwich ELISA in combination with streptavidin. In Western blotting after SDS-PAGE, HYB 212-01 reacts with biotinylated proteins in both reduced and non-reduced forms.
| Method | Usability | References |
| ELISA | Yes | |
| Immunoblotting | Yes | |
| Immunohistochemistry | Not determined |
1. Scott T & Eagleson M (1988) Concise Encyclopedia Biochemistry: Walter de Gruyter, New York.
