Anti-Pneumolysin
Mouse monoclonal antibody
Cat.No. HYB 041-01
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.13 M NaCl and 15 mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.
Pneumolysin (pneumococcal hemolysin) is an intracellular protein produced by all strains of S. pneumoniae (1) and is a cytoplasmic cholesterol-dependent cytolysin forming large pores in cholesterol-containing membranes and are therefore cytotoxic to mammalian cells (2). The molecular weight of Pneumolysin is approximately 53 kDa.
Pneumolysin
HYB 041-01 reacts specifically with Pneumolysin from at least 9 different types of pneumococcal strains when tested by SDS-PAGE and WB. The tested pneumococcal types were 5, 6A, 11A, 11F, 12B, 15F, 19A, 25F and 35A.
Not determined
HYB 041-01 reacts well in ELISA with coated pneumococcal extract. Excellent detection of pneumolysin is seen on WB from SDS-PAGE separated pneumococcal strains.
| Method | Usability | References |
| ELISA | Yes | |
| Immunoblotting | Yes | |
| Immunohistochemistry | Not determined |
1. Kalin M, Kanclerski K, Granstrom M, Mollby R (1987) Diagnosis of Pneumococcal Pneumonia by Enzyme-Linked Immunosorbent Assay of Antibodies to Pneumococcal Hemolysin (Pneumolysin). J Clin Microbiol 25:226-229.
2. Gilbert RJC (2002) Pore-forming toxins. Cell Mol Life Sci 59:832-844.
