Anti-Influenza B virus nucleoprotein, biotinylated
Mouse monoclonal antibody
Cat.No. HYB 116-03B
Preparation: Biotinylated
Content: 100 μL, 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15 mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.
Influenza viruses are common and highly infectious human pathogens. They are constantly mutating to modify their antigenicity and avoid elimination by immunity to previous generations of virus. The nucleoprotein of influenza B virus is a basic, phosphorylated multimeric protein consisting of 560 aa subunits that encapsidate the viral RNA genome to form a ribonucleoprotein particle (1).
Unpurified influenza B virus (B/Lee/40) for primary intranasal immunization, boosted intravenously with purified influenza B virus disrupted with Triton X-100 for 40 min at 37°C (2).
HYB 116-03 reacts with influenza B virus nucleoprotein as demonstrated by immunoblotting after SDS-PAGE. HYB 116-03 does not cross-react with influenza A virus (2).
Epitope differs from HYB 116-01 and HYB 116-02 as determined by inhibition ELISA.
Biotinylated HYB 116-03 is suitable as detecting antibody in combination with HYB 116-02 or HYB 116-01 + -02 as capture antibody in sandwich ELISA for influenza B virus (2).
HYB 116-03 reacts with influenza B infected, acetone-fixed Vero in immunofluorescence cytochemistry.
In Western blotting a dilution guideline of 1/40 has proved successful (2).
| Method | Usability | References |
| ELISA | Yes | 2 |
| Immunoblotting | ||
| Immunoflourescence |
1. Portela A, Digard P (2002) The influenza virus nucleoprotein: a multifunctional RNA-binding protein pivotal to virus replication. J Gen Virol 83:723-734
2. Glikmann G, Chen S-N, Mordhorst CH, Koch C (1995) Monoclonal antibodies for the rapid diagnosis of influenza-B virus infections by ELISA: production and characterization. Clin Diagn Virol 4:27-42.
