Anti-Influenza B virus nucleoprotein
Mouse monoclonal antibody
Cat.No. HYB 116-03B
Preparation: Biotinylated
Content: 100 µL, 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
Influenza viruses are common and highly infectious human pathogens. They are constantly mutating to modify their antigenicity and avoid elimination by immunity to previous generations of virus. The nucleoprotein of influenza B virus is a basic, phosphorylated multimeric protein consisting of 560 aa subunits that encapsidate the viral RNA genome to form a ribonucleoprotein particle (1)
Unpurified influenza B virus (B/Lee/40) for primal intranasal immunization, boosted intravenously with purified influenza B virus disrupted with Triton X-100 for 40 min at 37ºC (2)
HYB 116-03 reacts with influenza B virus nucleoprotein. as demonstrated by immunoblotting after SDS-PAGE
HYB 116-03 does not cross-react with influenza A virus (2)
Epitope differs from HYB 116-01 and HYB 116-02 as determined by inhibition ELISA.
Biotinylated HYB 116-03 is suitable as detecting antibody in combination with HYB 116-02 or HYB 116-01 + -02 as capture antibodu in sandwich ELISA for influenza B virus (2)
HYB 116-01 reacts with influenza B-infected, acetone-fixed Vero in immunoflourescence cytochemistry
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 2 | |
| Immunoblotting | |||
| Immunoflourescence |
1. Portela A, Digard P (2002) The influenza virus nucleoprotein: a multifunctional RNA-binding protein pivotal to virus replication. J Gen Virol 83:723-734
2. Glikmann G, Chen S-N, Mordhorst CH, Koch C (1995) Monoclonal antibodies for the rapid diagnosis of influenza-B virus infections by ELISA: production and characterization. Clin Diagn Virol 4:27-42.
