Anti-Procollagen type I C-terminal propeptide (human, PICP)
Mouse monoclonal antibody
Cat.No. BTE 002-18
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL volumes, 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
Type I collagen is present in connective tissue and bone where it makes up about 90% of the organic matrix. Type I collagen is produced from type-I procollagen by the proteolytic cleavage of N- and C-telopeptides, which are released into the circulation. The propeptides can be measured in serum as markers of the production of trype-I collagen. Elevated values of these propeptides are found in serum from patients with various metabolic or malignant bone diseases. Procollagen type I C-terminal propeptide (PICP) has a molecular mass of approximately 100 kDa. Its normal concentration in serum depends on age and sex (1).
PICP purified from second trimester amniotic fluid by affinity chromatography
BTE 002-18 is specific for for native PICP from amniotic fluid. Crossreactivity to other species is not determined.
Epitope differs from that of BTE 002-08
BTE 002-18 is especially well suited as detecting antibody in a sandwich ELISA in combination with BTE 002-08.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1/3000 | |
| Immunoblotting | Yes | ||
| Immunohistochemistry | Not determined |
The dilution guideline for ELISA is based on sandwich ELISA in combination with a polyclonal antibody against the antigen. Users should determine the optimal dilutions for their own purpose.
1. Hertel NT, Stoltenberg M, Juul A, Main KM, Müller J, Nielsen CT, Lorenzen I, Skakkebaek NE (1993) Serum concentrations of type I and III procollagen propeptides in healthy children and girls with central precocious puberty during treatment with gonadotropin-releasing hormone analog and cyproterone acetate. J Clin Endocrinol Metab 76:924-927.
