Anti-Procollagen type I C-terminal propeptide (human, PICP)
Mouse monoclonal antibody
Cat.No. BTE 002-18
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.
Type I collagen is present in connective tissue and bone where it makes up about 90% of the organic matrix. Type I collagen is produced from type-I procollagen by the proteolytic cleavage of N- and C-telopeptides, which are released into the circulation. The propeptides can be measured in serum as markers of the production of trype-I collagen. Elevated values of these propeptides are found in serum from patients with various metabolic or malignant bone diseases. Procollagen type I C-terminal propeptide (PICP) has a molecular mass of approximately 100 kDa. Its normal concentration in serum depends on age and sex (1).
PICP purified from second trimester amniotic fluid by affinity chromatography
BTE 002-18 is specific for for native PICP from amniotic fluid. Crossreactivity to other species is not determined.
Epitope differs from that of BTE 002-08
BTE 002-18 is especially well suited as detecting antibody in a sandwich ELISA in combination with BTE 002-08.
| Method | Usability | References |
| ELISA | Yes | |
| Immunoblotting | Yes | |
| Immunohistochemistry | Not determined |
1. Hertel NT, Stoltenberg M, Juul A, Main KM, Müller J, Nielsen CT, Lorenzen I, Skakkebaek NE (1993) Serum concentrations of type I and III procollagen propeptides in healthy children and girls with central precocious puberty during treatment with gonadotropin-releasing hormone analog and cyproterone acetate. J Clin Endocrinol Metab 76:924-927.
