Anti-Pregnancy associated plasma protein A (human, PAPP-A)
Mouse monoclonal antibody
Cat.No. ABS 006-24
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.
Pregnancy-associated plasma protein-A (PAPP-A, Insulin-like growth factor binding protein-4 protease, PAPP-A-proMBP complex) is a large heterotetrameric glycoprotein of approximately 500 kDa, which was first discovered in serum from pregnant women. The hetreotetramer consists of two PAPP-A subunits and two proMBP subunits. Low maternal serum levels of PAPP-A in first trimester biochemical screening is used as a marker of Down’s syndrome (trisomy 21).
Purified human PAPP-A adsorbed onto aluminum hydroxide
ABS 006-24 is specific for native human PAPP-A. Heat and SDS denatured PAPP-A is also recognized, but not acid treated PAPP-A .
The epitope of ABS 006-24 is different from BTE 004-09 and ABS 006-01. The epitope is located on the PAPP-A subunit of the heterotetramer.
BTE 004-09 as coating antibody and biotinylated ABS 006-24 as detection antibody is a good combination for sandwich ELISA.
ABS 006-24 only detecs unreduced PAPP-A in Western Blotting.
ABS 006-24 gives the strongest singal in IHC compared to ABS 006-01 and BTE 004-09.