Anti-neutrophil gelatinase-associated lipocalin (rat, NGAL)
Mouse monoclonal antibody, biotinylated
Cat.No. ABS 039-32B
Preparation: Biotinylated
Content: 50 μL, 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15 mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.
Rat NGAL, so called because it is the ortholog of human neutrophil gelatinase-associated lipocalin (NGAL), is also called lipocalin 2, 25-kDa alpha 2-microglobulin-related protein or neu-related lipocalin. It is expressed by neutrophils and various epithelial cells, including tubular cells of the kidney. It is involved in physiological processes such as the binding of siderophore iron and tissue growth and regeneration. It is up-regulated in a variety of pathological situations including infection, inflammation, renal injury and certain adenocarcinomas
Recombinant rat NGAL precursor adsorbed onto aluminum hydroxide gel
ABS 039-32 binds rat NGAL and shows no cross-reaction with human NGAL.
Not determined.
ABS 039-32 binds free rat NGAL in solution. ABS 039-32 (as biotinylated detection antibody) forms a sandwich ELISA pair with ABS 039-15 (as capture antibody) for measuring rat NGAL, (measuring range 4-400 pg/mL in an optimized assay).
| Method | Usability | References |
| ELISA | Yes | |
| Immunoblotting | Not determined | |
| Immunohistochemistry | Not determined |
1. Mishra J, Ma Q, Prada A, Mitsnefes M, Zahedi K, Yang J, Barasch J, Devarajan P (2003) Identification of neutrophil gelatinase-associated lipocalin as a novel early urinary biomarker for ischemic renal injury. J Am Soc Nephrol 14:2534-43.
Downloads
ABS 039-32B.pdf (30.23 kB)
Product specification
Pharma NGAL flyer.pdf (726.56 kB)
Pharma NGAL flyer
Animal NGAL Scientific references flyer.pdf (195.65 kB)
Animal NGAL Scientific references flyer
