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Anti-neutrophil gelatinase-associated lipocalin (rat, NGAL)

Mouse monoclonal antibody

Cat.No. ABS 039-32

Subclass IgG1/k

Presentation:

Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL volumes, 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC

Antigen:

Rat lipocalin-2 is also called rat NGAL as it is the ortholog of human neutrophil gelatinase-associated lipocalin (NGAL). Rat NGAL is a 25-kDa alpha 2-microglobulin-related protein or neu-related lipocalin. It is expressed by neutrophils and various epithelial cells, including cells of the renal proximal tubules. It is involved in physiological processes such as the binding of siderophore iron and tissue growth and regeneration. It is upregulated in a variety of pathological situations including infection, inflammation, ischemia-reperfusion and in certain adenocarcinomas

Immunogen:

Recombinant rat NGAL precursor adsorbed onto aluminum hydroxide gel

Specificity:

ABS 039-32 binds rat NGAL and shows no cross-reaction with human NGAL.

Epitope specificity:

Not determined

Reactivity:

ABS 039-32 binds free rat NGAL in solution. ABS 039-32 (as biotinylated detection antibody) forms a sandwich ELISA pair with ABS 039-08 (as capture antibody) for measuring rat NGAL, giving a detection limit of 0.012 ng/mL in an unoptimized buffer assay.

Application:

Method	Usability	Dilution guideline	References
ELISA	Yes	1/4000
Immunoblotting	Yes
Immunohistochemistry	Not determined

Application Remarks:

The dilution guideline for ELISA is based on use as detection antibody for antigen coated at 0.1-1 µg/ml. Users should determine the optimal dilutions for their own purposes.

References:

1. Mishra J, Ma Q, Prada A, Mitsnefes M, Zahedi K, Yang J, Barasch J, Devarajan P (2003) Identification of neutrophil gelatinase-associated lipocalin as a novel early urinary biomarker for ischemic renal injury. J Am Soc Nephrol 14:2534-43.

LCN2 protein 24p3 protein lipocalin 2 protein neu-related lipocalin protein Neutrophil gelatinase-associated lipocalin precursor protein NGAL protein oncogene 24p3 protein uterocalin protein siderocalin
Rat NGAL
Rat NGAL ELISA Kit (KIT 046)