Anti-neutrophil gelatinase-associated lipocalin (rat, NGAL)
Mouse monoclonal antibody
Cat.No. ABS 039-15
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.
Rat NGAL, so called because it is the ortholog of human neutrophil gelatinase-associated lipocalin (NGAL), is also called lipocalin 2, 25-kDa alpha 2-microglobulin-related protein or neu-related lipocalin. It is expressed by neutrophils and various epithelial cells, including tubular cells of the kidney. It is involved in physiological processes such as the binding of siderophore iron and tissue growth and regeneration. It is up-regulated in a variety of pathological situations including infection, inflammation, renal injury and certain adenocarcinomas.
Recombinant rat NGAL precursor with C-terminal His-tag coupled to carrier
ABS 039-15 binds rat NGAL and shows no cross-reaction with human NGAL.
Not determined
ABS 039-15 binds free rat NGAL in solution. ABS 039-15 (as capture antibody) forms a sandwich ELISA pair with ABS 039-32 (as biotinylated detection antibody) for measuring rat NGAL (measuring range 4-400 pg/mL in an optimized assay).
| Method | Usability | References |
| ELISA | Yes | |
| Immunoblotting | Not determined | |
| Immunohistochemistry | Not determined |
1. Mishra J, Ma Q, Prada A, Mitsnefes M, Zahedi K, Yang J, Barasch J, Devarajan P (2003) Identification of neutrophil gelatinase-associated lipocalin as a novel early urinary biomarker for ischemic renal injury. J Am Soc Nephrol 14:2534-43.
