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Anti-neutrophil gelatinase-associated lipocalin (rat, NGAL)

Mouse monoclonal antibody

Cat.No. ABS 039-08

Subclass IgG1/k

Presentation:

Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.

Antigen:

Rat NGAL, so called because it is the ortholog of human neutrophil gelatinase-associated lipocalin (NGAL), is also called lipocalin-2, 25-kDa alpha 2-microglubin-related protein or neu-related lipocalin. It is expressed by neutrophils and various epithelial cells, including cells tubular cells of the kidney. It is involved in physiological processes such as the binding of siderophore iron and tissue growth and regeneration. It is up-regulated in a variety of pathological situations including infection, inflammation, renal injury and certain adenocarcinomas.

Immunogen:

Recombinant rat NGAL precursor adsorbed onto aluminum hydroxide gel

Specificity:

ABS 039-08 binds rat NGAL and shows no cross-reaction with human NGAL.

Epitope specificity:

Not determined

Reactivity:

ABS 039-08 binds free rat NGAL in solution.

Application:

Method	Usability	References
ELISA	Yes
Immunoblotting	Not determined
Immunohistochemistry	Yes	2

References:

1. Mishra J, Ma Q, Prada A, Mitsnefes M, Zahedi K, Yang J, Barasch J, Devarajan P (2003) Identification of neutrophil gelatinase-associated lipocalin as a novel early urinary biomarker for ischemic renal injury. J Am Soc Nephrol 14:2534-43.

2. Zhang J, Ohm J,Vaidya V, Espandiari P, Knapton A,Goering P, Brown R, Bonventre J, Herman E (2009) NGAL Expression in Kidney of Spontaneously Hypertensive Rats (SHR) Treated with Doxorubicin (DXR) Alone or Combined with Dexrazoxane (DRZ) (Presented at Tox Expo 2009)

Lipocalin 2
Rat NGAL ELISA Kit (KIT 046)
KIT 046
Rat NGAL
NGAL

Larger Picture (123kB)

NGAL immunostaining in kidney, Formalin-fixed, paraffin-embedded tissue sections of cortical epithelial cells in the S1/S2 segments (proximal convoluted tubules) following DXR-induced acute kidney injury. Top panel, saline treatment; bottom panel, DXR treatment; X630 (2).