Anti-neutrophil gelatinase-associated lipocalin (pig, NGAL)
Mouse monoclonal antibody
Cat.No. ABS 048-28
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL volumes, 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
Pig neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2 or siderocalin) may, by analogy with its homologues in other mammalian species, be released from activated neutrophils in inflammation or infection, from certain epithelial cancers, and more dramatically, from renal tubular cells in response to ischemic or nephrotoxic injury.
Recombinant pig NGAL adsorbed onto aluminum hydroxide gel
ABS 048-28 binds pig NGAL
Not determined
ABS 048-28 binds free pig NGAL in solution.
ABS 048-28 (as biotinylated detection antibody) forms a sandwich ELISA pair with ABS 048-17 (as capture antibody) for measuring porcine NGAL.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1/20,000 | |
| Immunoblotting | Not determined | ||
| Immunohistochemistry | Not determined |
The dilution guideline for ELISA is based on use as detection antibody for antigen coated at 1 µg/ml. Users should determine the optimal dilutions for their own purposes.
