Preparation: Biotinylated
Content: 50 μL, 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15 mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.

Neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2, siderocalin and neutrophil lipocalin) is a member of the lipocalin family of proteins which bind and transport small lipophilic molecules. NGAL is released by activated neutrophils and occurs as 25-kDa glycosylated single protein chain monomers, which form dimers and small amounts of higher oligomers, as well as complexes with matrix metalloproteinase 9 (MMP-9; gelatinase B) (1). Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers (2). Expression of NGAL in the kidney is dramatically increased by acute kidney injury (3).

Human recombinant NGAL in monomer and dimer forms adsorbed to aluminium hydroxide gel

HYB 211-02 is specific for human NGAL, and cross-reacts with cynomolgus monkey (Macaca fascicularis) NGAL.

Epitope specificity differs from that of HYB 211-01.

HYB 211-02 reacts strongly with human NGAL. Strong reaction is seen when used as detection antibody in sandwich ELISA in combination with a polyclonal NGAL capture antibody and when tested on recombinant NGAL coated directly onto the well. HYB 211-02 reacts with NGAL in immunochemical staining of paraformaldehyde-fixed sections containing neutrophils. In Western blotting of SDS-PAGE gels, HYB 211-02 reacts with NGAL in both reduced and unreduced forms (4). A dilution guideline of 1/50 has proved successful (4).

HYB 211-02 (as biotinylated detection antibody) forms a sandwich pair with ABS 038-23 (as capture antibody) for measuring cynomolgus monkey (Macaca fascicularis) and human NGAL.