Anti-Neutrophil gelatinase-associated lipocalin (human, NGAL)
Mouse monoclonal antibody
Cat.No. HYB 211-01
Preparation: Protein-A/G purified
Content: 50 μL, 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent:0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.
Neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2, siderocalin and neutrophil lipocalin) is a member of the lipocalin family of proteins which bind and transport small lipophilic molecules. NGAL is released by activated neutrophils and occurs as 25-kDa glycosylated single protein chain monomers, which form dimers and small amounts of higher oligomers, as well as complexes with matrix metalloproteinase 9 (MMP-9; gelatinase B) (1). Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers (2). Expression of NGAL in the kidney is dramatically increased by acute kidney injury (3).
Human recombinant NGAL in monomer and dimer forms adsorbed to aluminium hydroxide gel
HYB 211-01 is specific for human NGAL. No cross-reaction with NGAL from other species has been detected.
Epitope specificity differs from that of HYB 211-02.
HYB 211-01 reacts strongly with human NGAL. Strong reaction is seen when used as detection antibody in sandwich ELISA in combination with a polyclonal NGAL capture antibody and when tested on recombinant NGAL coated directly onto the well. HYB 211-01 reacts with NGAL in immunochemical staining of paraformaldehyde-fixed sections containing neutrophils. In Western blotting of SDS-PAGE gels, HYB 211-01 reacts with NGAL in both reduced and unreduced forms (4). A dilution guideline of 1/500 has proved successful (4).
| Method | Usability | References |
| ELISA | Yes | 4 |
| Immunoblotting | Yes | 4 |
| Immunohistochemistry | Yes | 4,5 |
1. Kjeldsen L, Johnsen AH, Sengeløv H, Borregaard N (1993) Isolation and primary structure of NGAL, a novel protein associated with human neutrophil gelatinase. J Biol Chem 268:10425-10432.
2. Nielsen BS, Borregaard N, Bundgaard JR, Timshel S, Sehested M, Kjeldsen L (1996) Induction of NGAL synthesis in epithelial cells of human colorectal neoplasia and inflammatory bowel diseases. Gut 38:414-420.
3. Matthaeus T, Schulze-Lohoff E, Ichimura T, Weber M, Andreucci M, Park KM, Alessandrini A, Bonventre JV (2001) Co-regulation of neutrophil gelatinase-associated lipocalin and matrixmetalloproteinase-9 in the postischemic rat kidney. J Am Soc Nephrol 12:787A.
4. Kjeldsen L, Koch C, Arnljots K, Borregaard N (1996) Characterization of two ELISAs for NGAL, a newly described lipocalin in human neutrophils. J Immunol Methods 198:155-164. 5.Tong Z, Kunnumakkara AB, Wang H, Matsuo Y, Diagaradjane P, Harikumar KB, Ramachandran V, Sung B, Chakraborty A, Bresalier RS, Logsdon,C, Aggarwal BB, Krishnan S, Guha S (2008) Neutrophil Gelatinase–Associated Lipocalin: A Novel Suppressor of Invasion and Angiogenesis in Pancreatic Cancer. Canc Res 68: 6100-6108.
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Normal pancreas tissue immunohistochemically stained by using the monoclonal NGAL antibody HYB 211-01 (5).
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Chronic pancreatitis tissue were immunohistochemically stained by using the monoclonal NGAL antibody HYB 211-01 (5).
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Tissue microarrays from human PaCa (MDACC) were immunohistochemically stained by usinga monoclonal NGAL antibody HYB 211-01 (5).
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