Anti-Neutrophil gelatinase-associated lipocalin (dog, cat, guinea pig, NGAL)
Mouse monoclonal antibody, biotinylated
Cat.No. ABS 047-14B
Preparation: Biotinylated
Content: 50µL, 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
Dog neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2 or siderocalin) may, by analogy with its homologues in other mammalian species, be released from activated neutrophils in inflammation or infection, from certain epithelial cancers, and more dramatically from renal tubular cells in response to ischemic or nephrotoxic injury.
Recombinant dog NGAL adsorbed onto aluminum hydroxide gel
ABS 047-17 binds dog, cat and guinea pig NGAL
Not determined
ABS 047-14 binds free dog, cat and guinea pig NGAL.
ABS 047-14 (as biotinylated detection antibody) forms a sandwich ELISA pair with ABS 047-17 (as capture antibody) for measuring dog NGAL, giving a detection limit of 5 pg/mL in an unoptimized buffer assay. This assay also detects NGAL in cat and guinea pig serum.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1/10,000 | |
| Immunoblotting | |||
| Immunohistochemistry |
