Anti-Acetylcholinesterase (human brain, AChE)
Mouse monoclonal antibody
Cat.No. HYB 111-05
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.
Acetylcholinesterase (AChE, EC.3.1.1.7.) is an enzyme located in the postsynaptic membrane and in the muscle endplates, where it hydrolyses the neurotransmitter acetylcholin. AChE from brain is a tetramer (G4-AChE) with a molecular mass of 320 kDa, AChE from erythrocytes is a dimer (G2-AChE) with a molecular mass of 170 kDa (1,2).
Brain acetylcholinesterase, human, purified, detergent solubilized and adsorbed onto aluminum hydroxide gel(1).
HYB 111-05 is specific for human acetylcholinesterase. No cross-reaction is seen with human BtChE, or with AChE from flounder or Torpedo marmorata.
Not determined
HYB 111-05 reacts with both G2-and G4-AChE (native detergent soluble) in ELISA and EAIA using the antibody as capture antibody. HYB 111-05 reacts 17 times stronger with human brain AChE than with human erythrocyte AChE.
| Method | Usability | References |
| ELISA | Yes | |
| Immunoblotting | Yes | |
| Immunohistochemistry | Not determined |
1. Sorensen K, Gentinetta R, Brodbeck U (1982) An amphiphile-dependent form of human brain caudate nucleus acetylcholinesterase: purification and properties. J Neurochem 39:1050-1060.
2. Massoulie J, Bon S (1982) The molecular forms of cholinesterase and acetylcholinesterase in vertebrates. Annu Rev Neurosci 5:57-106.
3. Liao J, Boschetti N, Mortensen V, Jensen SP, Koch C, Norgaard-Pedersen B, Brodbeck U (1994) Characterization of salt-soluble forms of acetylcholinesterase from bovine brain. J Neurochem 63:1446-1453.
4. Liao J, Mortensen V, Noregaard-Pedersen B, Koch C, Brodbeck U (1993) Monoclonal antibodies against brain acetylcholinesterases which recognize the subunits bearing the hydrophobic anchor. Eur J Biochem 215:333-340.
5. Aziz-Aloya R, Sternfeld M, Soreq H (1993) Promoter elements and alternative splicing in the human ACHE gene. Prog Brain Res 98:147-153.
6. Norgaard-Pedersen B, Hangaard J, Bjerrum OJ (1983) Quantitative enzyme antigen immunoassay of acetylcholinesterase in amniotic fluid. Clin Chem 29:1061-1064.
