Anti-Acetylcholinesterase (bovine brain, AChE)
Mouse monoclonal antibody
Cat.No. HYB 101-03
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.
Acetylcholinesterase (AChE) is an enzyme located in the postsynaptic membrane and in the muscle endplates, where it hydrolyses the neurotransmitter acetylcholin. AChE from brain is a tetramer (G4-AChE) with a molecular mass of 320 kDa, AChE from erythrocytes is a dimer (G2-AChE) with a molecular mass of 170 kDa.
Bovine G4-acetylcholinesterase, purified, detergent solubilized and adsorbed onto aluminum hydroxide gel(1)
HYB 101-03 is specific for native, heat denatured and SDS plus heat denatured AChE.
Not determined
HYB 101-03 reacts with AChE from bovine and cross-react with human brain or erythrocytes AChE in ELISA. No cross-reactivity is seen with human AChE in Western blotting. No cross-reactivity to BtChE from human serum, AChE from flounder body muscle or Torpedo marmorata.
| Method | Usability | References |
| ELISA | Yes | |
| Immunoblotting | Yes | |
| Immunohistochemistry | Not determined |
1. Sorensen K, Gentinetta R, Brodbeck U (1982) An amphiphile-dependent form of human brain caudate nucleus acetylcholinesterase: purification and properties. J Neurochem 39:1050-1060.
2. Massoulie J, Bon S (1982) The molecular forms of cholinesterase and acetylcholinesterase in vertebrates. Annu Rev Neurosci 5:57-106.
3. Liao J, Boschetti N, Mortensen V, Jensen SP, Koch C, Norgaard-Pedersen B, Brodbeck U (1994) Characterization of salt-soluble forms of acetylcholinesterase from bovine brain. J Neurochem 63:1446-1453.
4. Liao J, Mortensen V, Noregaard-Pedersen B, Koch C, Brodbeck U (1993) Monoclonal antibodies against brain acetylcholinesterases which recognize the subunits bearing the hydrophobic anchor. Eur J Biochem 215:333-340.
5. Aziz-Aloya R, Sternfeld M, Soreq H (1993) Promoter elements and alternative splicing in the human ACHE gene. Prog Brain Res 98:147-153.
