Anti-Peptide YY (human, PYY)
Mouse monoclonal antibody
Cat.No. ABS 029-01
Preparation: Protein-A purified
Content: Available in 200 µL and 1 mL volumes, 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
Peptide tyrosine-tyrosine (PYY) amide is a 36 amino-acid. PYY is a member of the pancreatic polypeptide (PP) family of peptides (neuropeptide Y (NPY), PYY and PP). While NPY is localized in neurons (both in the central and peripheral nervous system) and PP in the pancreatic islets, PYY is found in both neurons and the gut. PYY is released in response to food intake from the same endocrine cells (L cells) in the intestinal mucosa as the glucagon-like peptides and inhibits gall bladder secretion, gut motility and pancreatic secretion. These effects are similar to those of PP and overlap with the gut inhibitory activity of GLP-1.
Synthetic human PYY adsorbed onto aluminum hydroxide gel
ABS 029-01 binds human PYY. Cross-reactivity with NPY or PP in solution is <0.1%.
Not determined
ABS 029-01 binds free PYY in solution.
ABS 029-01 reacts in ELISA with PYY coated directly onto the microtiter well.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1/16,000 | |
| Immunoblotting | Not determined | ||
| Immunohistochemistry | Not determined |
The dilution guideline for ELISA is based on use as detection antibody for antigen coated at 1µg/ml. Users should determine the optimal dilutions for their own purposes.
