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Anti Glucagon-like peptide-1 (GLP-1 (7-36) amide)

Mouse monoclonal antibody, biotinylated

Cat.No. HYB 147-06B

Subclass IgG1/k

Presentation:

Preparation: Biotinylated
Content: 50 無, 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8慢

Antigen:

Glucagon-like peptide 1(7-36)amide (GLP-1 (7-36)amide) is the principal active form of GLP-1, the other being GLP-1 (7-37). GLP-1 is a peptide hormone of the glucagon family, produced by the L cells of the intestinal mucosa from the same prohormone as glucagon. The active forms are potent stimulators of glucose-dependent insulin secretion. The sequence of GLP-1 is fully conserved in all mammalian species examined so far

Immunogen:

Synthetic GLP-1 (7-36)amide coupled to carrier and adsorbed onto aluminum hydroxide gel

Specificity:

HYB 147-06 is specific for the amidated C-terminus of the peptide and does not react with GLP-1(7-37) (1)

Epitope specificity:

C-terminal epitope of GLP-1(7-36)amide

Reactivity:

HYB 147-06 reacts with the amidated C-terminus of GLP-1(7-36)amide, GLP-1(9-36)amide and GLP-1(1-36)amide. HYB 147-06 can be used as capture antibody in sandwich ELISA (1) using HYB 147-12B or ABS 033-10 as detection antibody. HYB 147-06 has been used for the immunoblockade of endogenous GLP-1 in rats (2).

ABS 044-49 (as capture antibody) binds to the N-terminus of GLP-1(9-36)amide in a different manner from ABS 044-53, permitting some concomitant C-terminal binding of HYB 147-06B (as biotinylated detection antibody).

Application:

Method	Usability	Dilution guideline	References
ELISA		1/4000
Immunoblotting
Immunohistochemistry

Application Remarks:

The dilution guideline for ELISA is based on use as detection antibody for antigen coated at 0.1-1 痢/mL. Users should determine the optimal dilutions for their own purposes.

References:

1. Ghiglione M, Uttenthal LO, Koch C (1993) Monoclonal antibodies to glucagon-like peptide-1. Digestion 54:396-397.
2. van Delft J, Uttenthal O, Koch C, Ghiglione M (1999) Immunoblockade of endogenous glucagon-like peptide-1 by monoclonal antibodies in conscious rats: effect on the insulin response to intragastric glucose. Metabolism 48:41-46.