Anti-Glucagon-like peptide-1 (GLP-1, Non-amidated (-Arg-Gly), C-terminal specific)
Mouse monoclonal antibody
Cat.No. ABS 046-03
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL volumes, 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
GLP-1(7-36) amide is the principal active form of GLP-1, the other being GLP-1(7-37). GLP-1 is a peptide hormone of the glucagon family, produced by the L cells of the intestinal mucosa from the same prohormone as glucagon. The active forms are potent stimulators of glucose-dependent insulin secretion. The sequence of GLP-1 is fully conserved in all mammalian species examined so far.
Synthetic GLP-1(9-37) adsorbed onto aluminum hydroxide gel
ABS 046-03 binds GLP-1(1-37), GLP-1(7-37) and GLP-1(9-37).
Cross-reaction with C-terminally amidated GLP-1 is 0-16% in various circumstances.
C-terminus of GLP-1(1-37), GLP-1(7-37) and GLP-1(9-37)
ABS 046-03 binds free GLP-1(1-37), GLP-1(7-37) and GLP-1(9-37) in solution.
Two different ELISA sandwich setups can be made for measuring non-amidated GLP-1 forms: The first uses ABS 046-03 as a capture antibody with ABS 033-10B as a biotinylated detection antibody cross-reacting about 5% with C-terminally amidated GLP-1. The second combination uses ABS 046-03B as a biotinylated detection antibody and HYB 147-12 as a capture antibody cross- reacting about 16% with C-terminally amidated GLP-1. Results show detection limits of 44pmol/L which is 10-20 times higher than the basal concentration of GLP-1, so the assays have to be optimized.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1/1000 | |
| Immunoblotting | Not determined | ||
| Immunohistochemistry | Not determined |
The dilution guideline for ELISA is based on use as detection antibody for antigen coated at 0.1-1 µg/ml. Users should determine the optimal dilutions for their own purposes.
Downloads
ABS 046-03.pdf (30.07 kB) Product specification
GLP-1 and Exendin-4 brochure.pdf (414.39 kB)
GLP-1 and Exendin-4 brochure
