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Anti-Glucagon-like peptide-1 (GLP-1(9-37) and GLP-1(9-36)amide, N-terminal specific)

Mouse monoclonal antibody

Cat.No. ABS 044-53

Subclass IgG1/k

Presentation:

Preparation: Protein-A purified
Content: Available in 200 μL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.

Antigen:

Glucagon-like peptide-1(9-36)amide and GLP-1(9-37) are the forms of GLP-1 that result from the rapid degradation of the active forms of the peptide (GLP-1(7-36)amide and GLP-1(7-37)) by the enzyme dipeptidyl peptidase-IV (DPP-IV, also known as CD26 or adenosine deaminase binding protein). GLP-1 is a peptide hormone of the glucagon family, produced by the L cells of the intestinal mucosa from the same prohormone as glucagon. The active forms are potent stimulators of glucose-dependent insulin secretion. The sequence of GLP-1 is fully conserved in all mammalian species examined so far.

Immunogen:

Synthetic GLP-1(9-18) adsorbed onto aluminum hydroxide gel

Specificity:

ABS 044-53 binds human GLP-1(9-36)amide
Based on inhibition ELISA, ABS 044-53 cross-reacts <2% with GLP-1(7-36)amide

Epitope specificity:

The free N-terminus of GLP-1(9-36)amide and GLP-1(9-37)

Reactivity:

ABS 044-53 binds free GLP-1(9-36)amide in solution. ABS 044-53 has higher affinity for GLP-1(9-36)amide than ABS 044-49 as determined by inhibition.

Application: