Preparation: Protein-A/G purified

Content: Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Storage: 4-8ºC without exposure to light. No precautions necessary during handling.

Glucagon-like peptide 1(7-36)amide (GLP-1(7-36)amide) is the principal active form of GLP-1, the other

being GLP-1(7-37). GLP-1 is a peptide hormone of the glucagon family, produced by the L cells of the

intestinal mucosa from the same prohormone as glucagon. The active forms are potent stimulators of

glucose-dependent insulin secretion. The sequence of GLP-1 is fully conserved in all mammalian

species examined so far.

Synthetic GLP-1(7-17) adsorbed onto aluminum hydroxide gel

ABS 033-10 binds the free N-terminus of GLP-1(7-37) and GLP-1(7-36)amide and shows <0.2%

cross-reactivity with GLP-1(9-36)amide, glucagon and human GIP. Cross-reacts 0.25% with

GLP-1(1-37), approximately 6% with human GLP-2 and approximately 50% with exendin-4.

Free N-terminus of GLP-1(7-37) and GLP-1(7-36)amide.

ABS 033-10 binds free GLP-1(7-37) and free GLP-1(7-36)amide in solution.

ABS 033-10 reacts in ELISA with GLP-1(7-37) coated directly onto the microtiter well.

ABS 033-10 binds free exendin-4 in solution and can function as a capture antibody for exendin-4.

ABS 033-10B can be used as a detection antibody in combination with ABS 046-03 as a capture

antibody in order to measure non-amidated GLP-1 forms and cross-reacting about 5% with

C-terminally amidated GLP-1. Results show detection limits of 44pmol/L which is 10-20 times higher

than the basal concentration of GLP-1, so the assays have to be optimized.