BioPorto Diagnostic

AntibodyShop

Anti-Exendin-4

Mouse monoclonal antibody

Cat.No. ABS 012-23

Subclass IgG1/k

Presentation:

Preparation: Protein-A purified
Content: Available in 200 無 and 1 mL volumes, 1 mg/mL

Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8慢

Antigen:

Exendin-4 is a 39-amino-acid peptide found in venom from the Gila monster Helicoderma suspectum (1). It is a member of the glucagon-secretin family of peptide hormones and neuropeptides. Exendin-4 is a potent agonist of the GLP-1 receptor and hence a potent stimulator of insulin secretion (2).

Immunogen:

Synthetic exendin-4 coupled to carrier and adsorbed onto aluminum hydroxide gel.

Specificity:

Specific for exendin-4. No cross-reactivity with GLP-1, GLP-2 (human) or glucagon coated on ELISA wells.

Epitope specificity:

The epitope is in the 9-39 region of the peptide, and the antibody cross-reacts strongly with exedin (9-39)amide

Reactivity:

ABS 012-23 binds to exendin-4 when coated on ELISA wells and reacts specifically with exendin-4 in solution giving a Ka of 7x10E8 in an inhibition ELISA. The binding between ABS 012-23 and exendin-4 is disrupted by 0,1 M glycin pH 3.

ABS 012-23 (as biotinylated detection antibody) forms a sandwich ELISA pair with ABS 033-10 (as capture antibody) for measuring exedin-4, giving a detection limit of as little as 10 pmol/L in an unoptimized buffer assay.

Application:

Method	Usability	Dilution guideline	References
ELISA	Yes	1/1600
Immunoblotting	Not determined
Immunohistochemistry	Not determined

Application Remarks:

The dilution guideline for ELISA is based on use as detection antibody on antigen 0.2 痢/mL coated directly onto the microtiter well. Users should determine the optimal dilutions for their own purposes.

References:

1. Eng J, Kleinman WA, Singh L, Singh G, Raufman JP (1992) Isolation and characterization of exendin-4, an exendin-3 analogue, from Heloderma suspectum venom. Further evidence for an exendin receptor on dispersed acini from guinea pig pancreas. J Biol Chem 267:7402-7405.
2. Goke R, Fehmann HC, Linn T, Schmidt H, Krause M, Eng J, Goke B (1993) Exendin-4 is a high potency agonist and truncated exendin-(9-39)-amide an antagonist at the glucagon-like peptide 1-(7-36)-amide receptor of insulin-secreting beta-cells. J Biol Chem 268:19650-19655.