Preparation: Protein-A/G purified

Content: Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15mM sodium azide

Storage: 4-8ºC without exposure to light. No precautions necessary during handling.

Mannan-binding lectin (MBL), also called mannose-binding lectin or protein, belongs to the C-type family of collectins, showing calcium-dependent binding to certain sugars (1). It consists of oligomers of triple-chain subunits and its binding and complement activating activities depend on its normal oligomerization. On binding to mannan-like microbial surface carbohydrates, MBL activates the complement system by means of its own lectin pathway, depending on the MBL-associated serine proteases (MASPs). Because of the presence of different structural and promoter alleles in the population, 12% or more of the population have low concentrations (<50ng/mL) of normally oligomerized, functional MBL in plasma or serum (2).

MBL purified from human donor plasma and adsorbed onto aluminum hydroxide.

HYB 131-10 is specific for MBL from human serum or plasma.

The epitope is thought to be on the carbohydrate recognition domain and differs from that of HYB 131-01 and HYB 131-11.

HYB 131-10 reacts strongly with MBL. Strong reaction is seen in ELISA with MBL coated directly onto the microtiter well. In Western blotting HYB 131-10 reacts with human MBL in both its oligomerized state and as single protein chain of 26 kDa. HYB 131-10 can be used to measure MBL in plasma or serum but is not specific for oligomerized MBL when used in combination with itself or other MBL antibodies.