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Anti Mannan-binding lectin (human MBL), biotinylated

Mouse monoclonal antibody

Cat.No. HYB 131-01B

Subclass IgG1/k

Presentation:

Preparation: Biotinylated
Content: 50 無, 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15mM sodium azide
Storage: In the dark at 4-8慢

Antigen:

Mannan-binding lectin (MBL) belongs to the C-type family of collectins. On specific binding to microbial surface carbohydrates, MBL activates the complement system by means of its own lectin pathway, depending on the MBL-associated serine proteases (MASPs). Investigations have shown that up to 12% of normal blood donors (1) have low (<50ng/mL) MBL plasma concentrations.

Immunogen:

Human MBL (2)

Specificity:

HYB 131-01 is specific for MBL from human serum or plasma

Epitope specificity:

Epitope specificity differs from that of HYB 131-10 and HYB 131-11. Epitope is near the carbohydrate-binding site.

Reactivity:

In Western blotting, HYB 131-01 reacts with human MBL both in its polymeric conformation and as a single subunit of molecular mass 26 kDa.
Used as detection antibody in sandwich ELISA with a mannan or HYB 131-01 coat, HYB 131-01 is selective for normally oligomerized MBL.
HYB 131-01 is also well suited for immunohistochemistry on human tissue samples, frozen or paraffin embedded, from liver and brain.

Application:

Method	Usability	Dilution guideline	References
ELISA	Yes	1:5000
Immunoblotting	Yes
Immunohistochemistry	Yes		5

Application Remarks:

References:

1. Steffensen R, Thiel S, Varming K, Jersild C, Jensenius JC (2000) Detection of structural gene mutations and promoter polymorphisms in the mannan-binding lectin (MBL) gene by polymerase chain reaction with sequence-specific primers. J Immunol Methods 241:33-42.
2. Kawasaki N, Kawasaki T, Yamashina I (1983) Isolation and characterization of a mannan-binding protein from human serum. J Biochem (Tokyo) 94:937-947.
3. Madsen J, Tornoe I, Nielsen O, Koch C, Steinhilber W, Holmskov U (2003) Expression and localization of lung surfactant protein A in human tissues. Am J Respir Cell Mol Biol 29:591-7.
4. Holmskov UL (2000) Collectins and collectin receptors in innate immunity. APMIS Suppl 100:1-59.
5. Harboe M, Garred P, Borgen M S, Stahl G L, Roos A, Mollnes T E (2006) Design of a complement mannose-binding lectin pathway-specific activation system applicable at low serum dilutions. Clin Exp Immunol 144:512-520.

Please view the MBL reference flyer on the right hand side, which includes a complete list of references using HYB 131-01.