Anti-Complement factor I (human)
Mouse monoclonal antibody
Cat.No. HYB 061-01
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL volumes, 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide
Storage: In the dark at 4-8ºC
Factor I, a serine protease, is a regulatory component of both the alternative and the classical complement pathway. Factor I is synthesized as a single chain precursor of 88 kDa, processed to yield the active form, composed of two disulfide-linked chains, the alpha-chain (50 kDa) and the beta-chain (38 kDa) (2). The concantration in plasma is 35 µg/ml (1).
Factor I isolated from human plasma adsorbed onto aluminum hydroxide gel
HYB 061-01 is specific for the alpha-chain of human factor I
Not determined
HYB 061-01 reacts strongly with factor I. A strong reaction is seen with normal human plasma when used in sandwich ELISA in combination with a polyclonal antibody against factor I as capture antbody (e.g. sheep anti human factor I from Serotec). In Western blotting after SDS-PAGE, HYB 061-01 reacts with factor I in non-reduced form.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1/125 | |
| Immunoblotting | Yes | ||
| Immunohistochemistry | Not determined |
The dilution guideline for ELISA is based on sandwich ELISA in combination with a polyclonal antibody against the antigen. Users should determine the optimal dilutions for their own purpose.
1. Morley BJ and Walport MJ (2000) The Complement FactsBook. Academic Press, London, UK.
2. Law SKA, Reid KBM (1988) Complement. In: In Focus (Ed. Male D) IRL Press: Oxford.
3. Rasmussen JM (1991) Inherited deficiencies of complement proteins with special reference to congenital deficiency of the regulatory protein factor I. APMIS Suppl 22:1-48.
Downloads
HYB 061-01.pdf (35.02 kB) Product specification
ELISA Kits and Monoclonal antibodies for the Complement system.pdf (318.66 kB) ELISA Kits and Monoclonal antibodies for the Complement system
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