Anti-Complement factor I (human)
Mouse monoclonal antibody
Cat.No. HYB 061-01
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.
Factor I, a serine protease, is a regulatory component of both the alternative and the classical complement pathway. Factor I is synthesized as a single chain precursor of 88 kDa, processed to yield the active form, composed of two disulfide-linked chains, the alpha-chain (50 kDa) and the beta-chain (38 kDa) (2). The concantration in plasma is 35 µg/ml (1).
Factor I isolated from human plasma adsorbed onto aluminum hydroxide gel
HYB 061-01 is specific for the alpha-chain of human factor I
Not determined
HYB 061-01 reacts strongly with factor I. A strong reaction is seen with normal human plasma when used in sandwich ELISA in combination with a polyclonal antibody against factor I as capture antbody (e.g. sheep anti human factor I from Serotec). In Western blotting after SDS-PAGE, HYB 061-01 reacts with factor I in non-reduced form.
| Method | Usability | References |
| ELISA | Yes | |
| Immunoblotting | Yes | |
| Immunohistochemistry | Not determined |
1. Morley BJ and Walport MJ (2000) The Complement FactsBook. Academic Press, London, UK.
2. Law SKA, Reid KBM (1988) Complement. In: In Focus (Ed. Male D) IRL Press: Oxford.
3. Rasmussen JM (1991) Inherited deficiencies of complement proteins with special reference to congenital deficiency of the regulatory protein factor I. APMIS Suppl 22:1-48.
Downloads
HYB 061-01.pdf (35.05 kB) Product specification
ELISA Kits and Monoclonal antibodies for the Complement system - flyer.pdf (637.76 kB) ELISA Kits and Monoclonal antibodies for the Complement system
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