Anti complement factor H (human, ß1H-globulin)
Mouse monoclonal antibody
Cat.No. GAU 018-03
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
Factor H is a regulatory factor of the alternative pathway of complement activation. Factor H is a glycoprotein consisting of a single polypeptide chain with a molecular mass of 150 kDa.
Human Factor H
GAU 018-03 is specific for complement factor H in human serum and plasma (1).
Epitope specificity differs from that of GAU 020-03 (1).
GAU 018-03 can be used as a capture antibody in sandwich ELISA with GAU 020-03 as detection antibody. This antibody pair specifically detects full length 150 kDa factor H and does not cross react with the truncated forms of factor H (1)
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:80,000 | 1, 2 |
| Immunoblotting | Yes | ||
| Immunohistochemistry | ND |
1. Oppermann M, Baumgarten H, Brandt E, Gottsleben W, Kurts C, Gotze O (1990) Quantitation of components of the alternative pathway of complement (APC) by enzyme-linked immunosorbent assays. J Immunol Methods 133:181-190
2. Oppermann M, Kurts C, Zierz R, Quentin E, Weber MH, Gotze O (1991) Elevated plasma levels of the immunosuppressive complement fragment Ba in renal failure. Kidney Int. 40:939-947
3. Oppermann M, Höpken U, Götze O (1992) Assessment of complement activition in vivo. Imm. pharm 24:119-134
Downloads
GAU 018-03.pdf (23.71 kB) Product specification
Complement flyer.pdf (422.22 kB)
Complement flyer
Related Articles
Antibody MSDS
April 23 2008
