BioPorto Diagnostic

AntibodyShop

Anti-Complement component C5 (human)

Mouse monoclonal antibody

Cat.No. HYB 029-02

Subclass IgG1/k

Presentation:

Preparation: Protein-A/G purified

Content: Available in 200 無 and 1 mL volumes, 1 mg/mL

Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide

Storage: In the dark at 4-8慢

Antigen:

C5 is part of the membrane attack complex (MAC) of the complement system. C5 is split into C5a og C5b by the C5 convertases from the classical and the alternative complement pathway and the C5b fragment initiates the assembly of the terminal MAC (C5b-C9) (1). The molecular mass of C5 is 190 kDa.

Immunogen:

C5 isolated from human plasma adsorbed onto aluminum hydroxide gel

Specificity:

HYB 029-02 is specific for human C5. No reaction is seen with plasma from patients deficient in C5.

Epitope specificity:

Epitope specificity differs from that of HYB 029-03 but slightly overlap as determined by inhibition ELISA.

Reactivity:

HYB 029-02 reacts strongly with C5. A strong reaction is seen with normal human plasma, and no reaction is seen with plasma from patients deficient in C5, when tested in sandwich ELISA in combination with a polyclonal antibody against C5 (eg. DAKO A 055). In Western blotting after SDS-PAGE, HYB 029-02 reacts with C5 in normal human plasma (PEG precipitated C5) in nonreduced form only and no reaction is seen with plasma from C5 deficient patients.

Application:

Method	Usability	Dilution guideline	References
ELISA	Yes	1/8000
Immunoblotting	Yes
Immunohistochemistry	Not determined

Application Remarks:

The dilution guideline for ELISA is based on use as detection antibody for antigen coated at 0.1-1 痢/ml. Users should determine the optimal dilutions for their own purposes.

References:

1. Law SKA, Reid KBM (1988) Complement. In: In Focus (Ed. Male D) IRL Press: Oxford.