Anti-Complement component C3 (rat)
Mouse monoclonal antibody
Cat.No. HYB 118-02
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.
C3 is a key component of the complement system, because the lectin, classical and alternative activation pathways merge at the C3 activation step.
C3 purified from rat serum adsorbed onto aluminum hydroxide gel
HYB 118-02 is specific for rat C3. No cross-reaction is seen when tested with human C3.
Not determined
HYB 118-02 reacts strongly with rat C3. Strong reaction is seen when tested in sandwich ELISA in combination with a polyclonal antibody against rat C3.
Administered to rats transplanted with guinea-pig hearts it prolongs xenograft survival.
| Method | Usability | References |
| ELISA | Yes | |
| Immunoblotting | Not determined | |
| Immunohistochemistry | Yes | 1, 2 |
1. Kemp E, Dieperink H, Leth P, Jensenius JC, Nielsen B, Lillevang ST, Salomon S, Steinbruchel D, Larsen S, Koch C, et al. (1994) Monoclonal antibodies to complement C3 prolong survival of discordant xenografts: guinea pig heart to rat transplantation. Transplant Proc 26:1011-1015.
2. Nielsen B, Steinbruchel DA, Lillevang ST, Salomon S, Kemp E (1994) Natural history of hamster heart rejection when transplanted to rat: a pathoanatomical study including immunohistochemistry. Transplant Proc 26:1024-1025.
Downloads
HYB 118-02.pdf (29.67 kB) Product specification
ELISA Kits and Monoclonal antibodies for the Complement system - flyer.pdf (637.76 kB) ELISA Kits and Monoclonal antibodies for the Complement system
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April 23 2008
