Anti Complement component C3d (human)
mouse monoclonal antibody
Cat.No. HYB 030-08
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide
Storage: In the dark at 4-8ºC
C3 is a key component of the complement system since classical and alternative activation pathways merge at the C3 activation step when C3 is split into C3a and C3b. The molecular mass of C3 is 185 kDa and it consists of two chains (110 kDa and 75 kDa) held together by disulfide bonds (1,2).
C3 isolated from human plasma
HYB 030-08 is specific for human C3d, a fragment of the alpha chain (3)
Epitope specificity differs from that of HYB 005-01, HAV 003-05 and HAV 004-01 but slightly overlap as determined by inhibition ELISA.
HYB 030-08 reacts strongly with C3. Strong reaction is seen in ELISA with C3 directly coated onto the microtiterwell, or when used as detection antibody in sandwich ELISA in combination with a polyclonal antibody against C3 (e.g. DAKO A 0062).
In Western blotting after SDS-PAGE, HYB 030-08 reacts with C3 in reduced as well as nonreduced forms.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:30000 | |
| Immunoblotting | Yes | ||
| Immunohistochemistry | Not determined |
1. Law SKA, Reid KBM (1988) Complement. In: In Focus (Ed. Male D) IRL Press: Oxford.
2. Morley BJ and Walport MJ (2000) The Complement FactsBook. Academic Press, London, UK.
3. Nagar B, Jones RG, Diefenbach RJ, Isenman DE, Rini JM (1998) X-ray crystal structure of C3d: a C3 fragment and ligand for complement receptor 2. Science 280:1277-1281.
Downloads
HYB 030-08.pdf (12.09 kB) Product specification
Complement flyer.pdf (422.22 kB) Complement flyer
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