Anti-Complement component C3a/C3a (desArg) (human), biotinylated
Mouse monoclonal antibody
Cat.No. GAU 017-01B
Preparation: Biotinylated
Content: 100 µL, 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ŗC
Complement C3a is an anaphylatoxin of 77 amino acid residues released by the action of the C3 convertases on the N-terminal of the alpha chain of C3. It is rapidly inactivated by serum carboxypeptidase N which removes the C-terminal arginine residue generating C3a (desArg).
Human C3a (1, 2)
Recognizes an epitope that is present on human C3, C3a and C3a (desArg) (2, 3).
Does not cross-react with C4a or C5a (2, 3).
GAU 017-01 recognizes different epitopes on the 9 kDa C3a than GAU 013-16 (2, 3). No reaction is seen with a synthetic octapeptide representing the C3a C-terminal (2).
GAU 017-01 can be used as a biotinylated detection antibody in sandwich ELISA with GAU 013-16 capture antibody. Does not inhibit the biological activity of C3a (4).
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1/40,000 | |
| Immunoblotting | |||
| Immunohistochemistry |
The dilution guideline for ELISA is based on use as detection antibody for C3a (desArg) coated at 1 µg/ml. Users should determine the optimal dilutions for their own purposes.
1. Oppermann M, Haubitz M, Quintin E, Götze O (1988) Complement activation in patients with renal failure as detected through the quantitation of fragments of the complement proteins C3, C5, and Factor B. Klin Wochenschr 66:857-864.
2. Nezlin R, Freywald A, Oppermann M (1993) Proteins separeted from human IgG molecules. Mol. Immunol. 30:935-940.
3. Oppermann M, Liebmann F, Götze O. (1987) Purification and quantification of human C3a anaphylatoxin using monoclonal antibodies. Complement 4:205-206
4. Puschel GP, Oppermann M, Muschol W, Gotze O, Jungermann K. (1989) Increase of glucose and lactate output and decrease of flow by human anaphylatoxin C3a but not C5a in perfused rat liver. FEBS Lett. 16;243(1):83-7.
5.Oppermann M, Kurts C, Zierz R, Quentin E, Weber MH, Gotze O (1991) Elevated plasma levels of the immunosuppressive complement fragment Ba in renal failure. Kidney Int. 40:939-947.
6. Ammon HPT, Ege W, Oppermann M, Göpel W, Eisele S (1995) Improvement in the long-term stability of an amperometric glucose sensor system by Introducing a cellulose membrane of bacterial origin. Anal. chem. 67:466-471.
7. Lhotta K, Würzner R, Kronenberg F, Oppermann M, König P (1998) Rapid activation of the complement system by cuprophane depends on complemet component C4. Kidney Int. 53:1044-1051.
Downloads
GAU 017-01B.pdf (31.33 kB) Product specification
Elisa kits and Monoclonal antibodies for the Complement system flyer.pdf (478.39 kB)
ELISA Kits and Monoclonal antibodies for the Complement system
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