Anti-Complement component C3a/C3a (desArg) (human)
Mouse monoclonal antibody
Cat.No. GAU 013-16
Preparation: Protein-A purified
Content: Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage: 4-8şC without exposure to light. No precautions necessary during handling.
Complement C3a is an anaphylatoxin of 77 amino acid residues released by the action of the C3 convertases on the N-terminal of the alpha chain of C3. It is rapidly inactivated by serum carboxypeptidase N which removes the C-terminal arginine residue generating C3a (desArg).
Human C3a adsorbed onto aluminum hydroxide gel (1)
GAU 013-16 is specific for human C3, C3a and C3a (desArg) (1) and does not cross-react with C4a og C5a (2,3)
GAU 013-16 recognizes a different epitope on the 9 kDa C3a than GAU 017-01 (2, 3). No reaction is seen with a synthetic octapeptide representing the C3a C-terminal (2).
GAU 013-16 can be used as a capture antibody in sandwich ELISA with GAU 017-01 as a biotinylated detection antibody (4). GAU 013-16 can also be used to immunopurify C3a (5, 6). GAU 013-16 effectively inhibits the biological activitiy of C3a in a guinea-pig platelet activation assay (6).
| Method | Usability | References |
| ELISA | Yes | 1,2,6 |
| Immunoblotting | Yes | 2,6 |
| Immunohistochemistry | Not determined |
1. Oppermann M, Haubitz M, Quintin E, Götze O (1988) Complement activation in patients with renal failure as detected through the quantitation of fragments of the complement proteins C3, C5, and Factor B. Klin Wochenschr 66:857-864.
2. Nezlin R, Freywald A, Oppermann M (1993) Proteins separeted from human IgG molecules. Mol. Immunol. 30:935-940.
3. Oppermann M, Liebmann F, Götze O. (1987) Purification and quantification of human C3a anaphylatoxin using monoclonal antibodies. Complement 4:205-206
4.Oppermann M, Kurts C, Zierz R, Quentin E, Weber MH, Gotze O (1991) Elevated plasma levels of the immunosuppressive complement fragment Ba in renal failure. Kidney Int. 40:939-947.
5. Püschel GP, Hespeling U, Oppermann M, Dieter P (1993) Increase in prostanoid formation in rat liver macrophages (Kupffer cells) by human anaphylatoxin C3a. Hepatology. 18(6):1516-21.
6. Puschel GP, Oppermann M, Muschol W, Gotze O, Jungermann K. (1989) Increase of glucose and lactate output and decrease of flow by human anaphylatoxin C3a but not C5a in perfused rat liver. FEBS Lett. 16;243(1):83-7.
Downloads
GAU 013-16.pdf (31.02 kB) Product specification
ELISA Kits and Monoclonal antibodies for the Complement system - flyer.pdf (637.76 kB)
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April 23 2008
