Anti-Complement component C2 (human)
Mouse monoclonal antibody
Cat.No. HYB 050-08
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL volumes, 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide
Storage: In the dark at 4-8ºC
Complement component C2 is a component of the classical complement pathway and is involved in activation of C3 and C5. C2 consists of a single polypeptide chain with a molecular mass of 102 kDa (2).
Complement component C2 isolated from human plasma adsorbed onto aluminum hydroxide gel(1)
HYB 050-08 is specific for C2 from human plasma/serum. Cross-reactivity to C2 from other species has not been tested.
Epitope specificity differs from that of HYB 050-04 and HYB 050-05 as determined by inhibition ELISA.
HYB 050-08 reacts strongly with C2. A strong reaction is seen with human serum/plasma and no reaction is seen with serum from patients deficient in C2, when tested in sandwich ELISA with a polyclonal antibody against C2. In Western blotting after SDS-PAGE, HYB 050-08 reacts with C2 and a subfraction of C2 believed to be C2b, both under reducing and non-reducing conditions. In ELISA, reaction is also seen with C2 directly coated onto the microtiter well.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1/4000 | 1 |
| Immunoblotting | Yes | 1/250 | 1 |
| Immunohistochemistry | Not determined |
The dilution guideline for ELISA is based on sandwich ELISA in combination with a polyclonal antibody against the antigen. Users should determine the optimal dilutions for their own purpose.
1. Stenbaek EI, Koch C, Barkholt V, Welinder KG (1986) Human complement component C2: production and characterization of polyclonal and monoclonal antibodies against C2. Mol Immunol 23:879-886.
2. Law SKA, Reid KBM (1988) Complement. In: In Focus (Ed. Male D) IRL Press: Oxford.
Downloads
HYB 050-08.pdf (29.75 kB) Product specification
Elisa kits and Monoclonal antibodies for the Complement system flyer.pdf (478.39 kB) ELISA Kits and Monoclonal antibodies for the Complement system
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