Anti Complement component C2 (human)
mouse monoclonal antibody
Cat.No. HYB 050-05
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide
Storage: In the dark at 4-8ºC at 4-8ºC
Complement component C2 is a component of the classical complement pathway and is involved in activation of C3 and C5. C2 consists of a single polypeptide chain with a molecular mass of 102 kDa (2).
Complement component C2 isolated from human plasma (1)
HYB 050-05 is specific for C2 from human plasma/serum. Crossreactivity to C2 from other species has not been tested.
Epitope specificity differs from that of HYB 050-04 and HYB 050-08 as determined by inhibition ELISA.
HYB 050-05 reacts strongly with C2. Strong reaction is seen with human serum/plasma and when used as detecting antibody in sandwich ELISA in combination with a polyclonal antibody against C2. In Western blotting after SDS-PAGE, HYB 050-05 reacts with C2 and a subfraction of C2 believed to be C2b, but only under non-reducing conditions.
HYB 050-05 is suitable for Sepharose column purification of C2 from plasma (3,4).
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:4000 | 1 |
| Immunoblotting | Yes | 1:500 | 1 |
| Immunohistochemistry | Not determined |
1. Stenbaek EI, Koch C, Barkholt V, Welinder KG (1986) Human complement component C2: production and characterization of polyclonal and monoclonal antibodies against C2. Mol Immunol 23:879-886.
2. Law SKA, Reid KBM (1988) Complement. In: In Focus (Ed. Male D) IRL Press: Oxford.
3. Laich A, Moffatt B, Wong KHN, Hickling TP, Koch C, Sim RB (2001) Purification of second component of human complement, C2, by antibody affinity chromatography. Intern J Bio-Chromatography 6:151-162.
4. Laich A, Sim RB (2001) Complement C4bC2 complex formation: an investigation by surface plasmon resonance. Biochim Biophys Acta 1544:96-112.
Downloads
HYB 050-05.pdf (13.69 kB) Product specification
Complement flyer.pdf (422.22 kB) Complement flyer
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