Anti-Complement component C5a/C5a(desArg) anaphylatoxin (human)
Mouse monoclonal antibody
Cat.No. GAU 025-05
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage: 4-8şC without exposure to light. No precautions necessary during handling.
Complement C5a is generated in the circulation by the activation of complement. It is a so-called anaphylatoxin of 74 amino-acid residues generated by the action of C5 convertase, which cleaves C5 into C5a and C5b. It is rapidly inactivated by serum carboxypeptidase B, which removes the C-terminal arginine residue generating C5a(desArg) (1).
Purified human C5a
GAU 025-05 reacts with human C5a/C5a(desArg) and cross-reacts with baboon C5a/C5a(desArg).
GAU 025-05 binds to a neoepitope exposed on C5a/C5a(desArg) (1).
GAU 025-05 reacts well with C5a/C5a(desArg). A strong reaction is seen in ELISA with recombinant C5a coated onto the microtiter well.
| Method | Usability | References |
| ELISA | Yes | 1 |
| Immunoblotting | Yes | 1 |
| Immunohistochemistry | Not determined |
1. Oppermann M, Schulze M, Götze O (1991) A sensitive enzyme immunoassay for the quantitation of human C5a/C5a(desArg) anaphylatoxin using a monoclonal antibody with specificity for a neoepitope. Complement Inflamm. 8:13-24.
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GAU 025-05.pdf (29.71 kB) Product specification
ELISA Kits and Monoclonal antibodies for the Complement system - flyer.pdf (637.76 kB)
ELISA Kits and Monoclonal antibodies for the Complement system
