Anti-Surfactant protein D (human, hSP-D)
Mouse monoclonal antibody, biotinylated
Cat.No. HYB 246-04B
Preparation: Biotinylated
Content: 100 μL, 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15 mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.
Surfactant protein D (SP-D) is synthesized and secreted by lung epithelial cells. It belongs to group III of the family of C-type lectins and members of this group has overall structure consisting of multiple globular 'head' regions linked by triple-helical, collagen-like, strands. This group also includes SP-A and the serum proteins mannan-binding protein, conglutinin and collectin-43, all of which have been shown to bind to the C1q receptor found on a wide variety of cells. Both SP-D and SP-A have been shown to enhance oxygen radical production by alveolar macrophages. The serum concentration is approximately 700 ng/ml in healthy individuals (1,4).
Native human SP-D purified from late pregnancy amniotic fluid adsorbed onto aluminum hydroxide gel
HYB 246-04 is specific for SP-D from amniotic fluid, serum and bronchioalveolar lavage. HYB 246-04 does not influence SP-D’s binding to carbohydrates, but block binding to GP-340 (6).
Epitope is localised within the C-terminal neck CRD (carbohydrate recognition domain) of SP-D
HYB 246-04 is suitable as detection antibody in sandwich ELISA in combination with a polyclonal antibody against SP-D. Binding of HYB 246-04 is Ca++ dependent.
In Western blotting after SDS-PAGE HYB 246-04 reacts with non-reduced SP-D only. A dilution guideline of 1/2000 has proved successful (4).
| Method | Usability | References |
| ELISA | Yes | |
| Immunoblotting | ||
| Immunohistochemistry |
1. Holmskov UL (2000) Collectins and collectin receptors in innate immunity. APMIS Suppl 100:1-59.
2. Johansson J, Curstedt T (1997) Molecular structures and interactions of pulmonary surfactant components. Eur J Biochem 244:675-693.
3. Husby S, Herskind AM, Jensenius JC, Holmskov U (2002) Heritability estimates for the constitutional levels of the collectins mannan-binding lectin and lung surfactant protein D. A study of unselected like-sexed mono- and dizygotic twins at the age of 6-9 years. Immunology 106:389-394.
4. Leth-Larsen R, Nordenbaek C, Tornoe I, Moeller V, Schlosser A, Koch C, Teisner B, Junker P, Holmskov U (2003) Surfactant protein D (SP-D) serum levels in patients with community-acquired pneumonia. Clinical Immunology 108:29-37.
5. Leth-Larsen R, Garred P, Jensenius H, Meschi J, Hartshorn K, Madsen J, Tornoe I, Madsen OH, Sørensen G, Crouch E, Holmskov U (2005) A common Polymorphism in the SFTPD Gene Influences Assembly, Function, and Concentration of Surfactant Protein D. J Immunol 174:1532-1538.
6. Hartshorn KL, White, MR, Tecle T, Tornoe I, Sørnsen GL, Crouch EC, Holmskov U (2007) Reduced infulenza viral neutralizing activity of natural human trimers of surfactant protein D. Resp Res 8
