Anti Glycoprotein-340 (gp-340)
mouse monoclonal antibody
Cat.No. HYB 213-06
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide
Storage: In the dark at 4-8ºC
Glycoprotein-340 is member of the scavenger receptor superfamily and has been identified as a putative SP-D receptor. Gp-340 has been shown to bind SP-D in a calcium dependent manner through a protein-protein interaction. Like SP-D, gp-340 is mainly found in lung tissue and immunohistochemistry revealed high expression in alveolar macrophages (3). An identical protein is present in saliva (salivary agglutinin) and both forms are encoded by the gene DMBT1 (6).
Native gp-340 purified from bronchioaveolar lavage from proteinosis patients
HYB 213-06 is specific for gp-340
Not determined
In Western blotting after SDS-PAGE HYB 213-06 reacts strongly with gp-340 in non-reduced forms.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:16000 | |
| Immunoblotting | Yes | 1:1000 | 1, 5, 6 |
| immunohistochemistry | Yes | 1:100 | 1, 2, 4 |
1. Ligtenberg TJ, Bikker FJ, Groenink J, Tornoe I, Leth-Larsen R, Veerman EC, Nieuw Amerongen AV, Holmskov U (2001) Human salivary agglutinin binds to lung surfactant protein-D and is identical with scavenger receptor protein gp-340. Biochem J 359:243-248.
2. Bikker FJ, Ligtenberg AJ, van der Wal JE, van der Keijbus PA, Holmskov U, Veerman EC, Nieuw Amerongen AV (2002) Immunohistochemical detection of salvary agglutinin/gp-340 in human parotid, submandibular, and labial salivary glands. J Dent Res 81:134-139.
3. Holmskov U, Mollenhauer J, Madsen J, Vitved L, Gronlund J, Tornoe I, Kliem A, Reid KB, Poustka A, Skjodt K (1999) Cloning of gp-340, a putative opsonin receptor for lung surfacant protein D. Proc Natl Acad Sci USA 96:10794-10799.
4. Bisgaard HC, Holmskov U, Santoni-Rugiu E, Nagy P, Nielsen O, Ott P, Hage E, Dalhoff K, Rasmussen LJ, Tygstrup N (2002) Heterogeneity of ductular reactions in adult rat and human liver revealed by novel expression of deleted in malignant brain tumor 1. Am J Pathol 161:1187-1198.
5. Prakobphol A, Xu F, Hoang VM, Larsson T, Bergstrom J, Johansson I, Frangsmyr L, Holmskov U, Leffler H, Nilsson C, Boren T, Wright JR, Stromberg N, Fisher SJ (2000) Salivary agglutinin, which binds Streptococcus mutans and Helicobacter pylori, is the lung scavenger receptor cysteine-rich protein gp-340. J Biol Chem 275:39860-39866.
6. Bikker FJ, Ligtenberg AJ, Nazmi K, Veerman EC, van't Hof W, Bolscher JG, Poustka A, Nieuw Amerongen AV, Mollenhauer J (2002) Identification of the bacteria-binding peptide domain on salivary agglutinin (gp-340/DMBT1), a member of the scavenger receptor cysteine-rich superfamily. J Biol Chem 277:32109-32115.
7. Kang W, Nielsen O, Fenger C, Madsen J, Hansen S, Tornoe I, Eggleton P, Reid KB, Holmskov U (2002) The scavenger receptor, cysteine-rich domain-containing molecule gp-340 is differentially regulated in epithelial cell lines by phorbol ester. Clin Exp Immunol 130:449-458.
8. Wu Z, Lee S, Abrams W, Weissman D, Malamud D (2006) The N-Terminal SRCR-SID Domain of gp-340 Interacts with HIV Type 1 gp120 Sequences and Inhibits Viral Infection. AIDS Res Hum Retroviruses 22:508-15.
