Anti-Glycoprotein-340 (gp-340)
Mouse monoclonal antibody
Cat.No. HYB 213-01
Preparation: Protein-A purified
Content: Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.
Glycoprotein (gp)-340 is member of the scavenger receptor superfamily and has been identified as a putative SP-D receptor. Gp-340 has been shown to bind SP-D in a calcium-dependent manner through a protein-protein interaction (1). Like SP-D, gp-340 is found in the lungs and immunohistochemistry shows high expression in alveolar macrophages (1). An identical protein, salivary agglutinin, is present in saliva (2-4) and both gp-340 and salivary agglutinin are encoded by the gene DMBT1 (5-7).
Native gp-340 purified from bronchoalveolar lavage fluid from patients with pulmonary alveolar proteinosis, adsorbed onto aluminum hydroxide gel
HYB 213-01 Reacts with a carbohydrate (sialic acid) epitope and cross-reacts with salivary MUC7 in immunohistochemistry (4).
Carbohydrate (sialic acid) moiety
In Western blotting after SDS-PAGE HYB 213-01 reacts strongly with gp-340 in both reduced and non-reduced forms. Strong reaction is seen in ELISA with gp-340 directly coated onto the microtiter well. In Western blotting a dilution guideline of 1/200 and 1/10.000 has proved successful (1, 2, 3, 4, 6).
| Method | Usability | References |
| ELISA | Yes | |
| Immunoblotting | Yes | 1,2,3,4,6 |
| Immunohistochemistry | Yes | 1,5,6 |
1. Holmskov U, Lawson P, Teisner B, Tornoe I, Willis AC, Morgan C, Koch C, Reid KBM (1997) Isolation and characterization of a new member of the scavenger receptor superfamily, glycoprotein-340 (gp-340), as a lung surfactant protein-D binding molecule. J Biol Chem 272:13743-13749.
2. Prakobphol A, Xu F, Hoang VM, Larsson T, Bergstrom J, Johansson I, Frangsmyr L, Holmskov U, Leffler H, Nilsson C, Boren T, Wright JR, Stromberg N, Fisher SJ (2000) Salivary agglutinin, which binds Streptococcus mutans and Helicobacter pylori, is the lung scavenger receptor cysteine-rich protein gp-340. J Biol Chem 275:39860-39866.
3. Ligtenberg TJM, Bikker FJ, Groenink J, Tornoe I, Leth-Larsen R, Veerman EC, Nieuw Amerongen AV, Holmskov U (2001) Human salivary agglutinin binds to lung surfactant protein-D and is identical with scavenger receptor protein gp-340. Biochem J 359:243-248.
4. Bikker FJ, Ligtenberg AJ, van der Wal JE, van den Keijbus PA, Holmskov U, Veerman EC, Nieuw Amerongen AV (2002) Immunohistochemical detection of salivary agglutinin/gp-340 in human parotid, submandibular, and labial salivary glands. J Dent Res 81:134-139.
5. Holmskov U, Mollenhauer J, Madsen J, Vitved L, Gronlund J, Tornoe I, Kliem A, Reid KB, Poustka A, Skjodt K (1999) Cloning of gp-340, a putative opsonin receptor for lung surfactant protein D. Proc Natl Acad Sci USA 96:10794-10799.
6. Mollenhauer J, Herbertz S, Holmskov U, Tolnay M, Krebs I, Merlo A, Schrøder HD, Maier D, Breitling F, Wiemann S, Gröne HJ, Poustka A (2000) DMBT1 encodes a protein involved in the immune defense and in epithelial differentiation and is highly unstable in cancer. Cancer Res 60:1704-1710.
7. Bikker FJ, Ligtenberg AJ, Nazmi K, Veerman EC, van't Hof W, Bolscher JG, Poustka A, Nieuw Amerongen AV, Mollenhauer J (2002) Identification of the bacteria-binding peptide domain on salivary agglutinin (gp-340/DMBT1), a member of the scavenger receptor cysteine-rich superfamily. J Biol Chem 277:32109-32115.
Larger Picture (419kB)
Localization of gp-340 by immunohistochemical staining of paraffin embedded section of human lung with a streptavidin biotin immunocytochemical technique. The immunoreaction product, stained with HYB 213-01, is red and the section was counterstained with Mayers hematoxylin.
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April 23 2008
