Anti L-Ficolin (human)
Mouse monoclonal antibody
Cat.No. ABS 005-16
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL, 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
L-Ficolin (Ficolin-2) is an innate immunity pattern recognition molecule found in human serum. L-Ficolin binds to distinct pathogen-associated molecular patterns (PAMP), such as carbohydrates (GlcNAc), lipoteichoic acid and acetylated groups thereby facilitating phagocytosis and activation of complement through the lectin route using the same serine proteases as mannose-binding lectin (MBL) named MASP ((MBL)-associated serine protease) (1).
Purified procaryot expressed recombinant human L-Ficolin and full-length L-Ficolin (Ficolin-2) protein
ABS 005-16 reacts specifically with L-Ficolin. No cross reactivity is seen with M-Ficolin (Ficolin-1) and H-Ficolin (Ficolin-3)
Not determined
ABS 005-16 is especially well suited as capture antibody in a sandwich ELISA in combination with ABS 005-19. Furthermore, in ELISA, ABS 005-16 reacts with recombinant L-Ficolin coated directly into the microtiter well.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:2000 | 1 |
| Immunoblotting | Yes | 1 | |
| Immunohistochemistry | Not determined |
1. Munthe-Fog L, Hummelshøj T, Hansen BE, Koch C, Madsen HO, Skjødt K, Garred P (2007) The impact of FCN2 polymorphisms and haplotypes on the Ficolin-2 serum levels. Scand J Immunol 65:383-392.
