Anti Collectin-43 (bovine, CL-43)
Mouse monoclonal antibody
Cat.No. HYB 260-01
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide
Storage: In the dark at 4-8ºC
Collectin-43 is a serum protein produced by the liver and a member of the collectin family. Collectins bind to microbial surface carbohydrates in a calcium-dependent way and thereby impeding infectivity or mediating phagocytosis through specific receptors on the phagocytes. Collectin-43 has so far only been found in Bovidae. It was given its name from its reduced mass of 43 kDa.
Native CL-43 purified from bovine serum (4)
HYB 260-01 is specific for bovine CL-43. No cross-reaction is seen with conglutinin in Western blotting.
Not determined
In Western blotting after SDS-PAGE HYB 260-01 reacts strongly with non-reduced CL-43 and weaker with reduced CL-43.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:20000 | 5 |
| Immunoblotting | Yes | 1:100 | |
| Immunohistochemistry | Not determined |
1. Holmskov UL (2000) Collectins and collectin receptors in innate immunity. APMIS Suppl 100:1-59.
2. Rothmann AB, Mortensen HD, Holmskov U, Højrup P (1997) Structural characterization of bovine collectin-43. Eur J Biochem 243:630-635.
3. Reading PC, Holmskov U, Anders EM (1998) Antiviral activity of bovine collectins against rotaviruses. J Gen Virol 79:2255-2263.
4. Holmskov U, Teisner B, Willis AC, Reid KB, Jensenius JC (1993) Purification and characterization of a bovine serum lectin (CL-43) with structural homology to conglutinin and SP-D and carbohydrate specificity similar to mannan-binding protein. J Biol Chem 268:10120-25.
5. Hartshorn KL, Holmskov U, Hansen S, Zhang P, Meschi J, Mogues T, White MR, Crouch EC (2002) Distinctive anti-influenza properties of recombinant collectin 43. Biochem J 366:87-96.
