Anti Antithrombin III (human)
Mouse monoclonal antibody
Cat.No. HYB 230-04
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide
Storage: In the dark at 4-8ºC
Antithrombin III (AT III) is a single chain glycoprotein of molecular mass 58 kDa with four glycosylation sites and three intra-molecular disulfide bonds. AT III is normally present in human plasma at a concentration of approx. 12.5 mg/dl. It is the major inhibitor of thrombin. Inactivation of thrombin by AT-III occurs by formation of a covalent bond resulting in an inactive 1:1 stoichiometric complex between the two components.
Antithrombin III isolated from human plasma (1)
HYB 230-04 is specific for human AT III, native form
Epitope specificity differs from that of HYB 230-01, HYB 228-10 and HYB 228-12.
HYB 230-04 reacts strongly with native AT III. Strong reaction is seen in ELISA with native AT III coated directly onto the microtiter well, and also when used as detection antibody in sandwich ELISA in combination with a polyclonal capture antibody (eg. DAKO A 296). HYB 230-04 reacts with AT III in Western blotting after SDS-PAGE.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:32,000 | |
| Immunoblotting | Yes | ||
| Immunohistochemistry | Not determined |
1. Sorensen PJ, Ezban M, Jessen TE, Sas G, Blasko G, Jorgensen M (1990) Purification and characterisation of the pathological antithrombin III "Aalborg". Thromb Res 57:807-812.
