Anti Vitronectin (bovine)

Mouse monoclonal antibody

Cat.No. CSI 004-27

Subclass IgG1/k
Presentation:

Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC

Antigen:

Vitronectin is a plasma glycoprotein that circulates in the blood. Vitronectin is circulating as a mixture of both 75 kDa and 65 kDa forms. Vitronectin is a major cell adhesive glycoprotein and is a common component of extracellular matrix and plasma. It competes effectively with other plasma proteins and is often involved in cell attachment, regulation of blood coagulation and immune responses. It has similar tissue distribution to fibronectin and also its integrin receptor recognises fibronectin (1).

Immunogen:

Lysed bovine corneal endothelial cells and extracellular matrix

Specificity:

CSI 004-27 is highly specific for vitronectin. There is no evidence for cross-reactivity with other connective tissue proteins (fibronectin, elastin, collagen, laminin).
CSI 004-27 cross-reacts with sheep vitronectin, no reactivity with human or horse.

Epitope specificity:

Not determined

Reactivity:

CSI 004-27 is suitable for ELISA, immunoblotting and immunostaining of frozen PLP-fixed sections of bovine tissues. The antibody can be used as an affinity purification reagent of vitronectin from bovine plasma or serum and to quantitatively deplete plasma or serum of vitronectin. It can also be used to probe vitronectin conformation.

Application:
Method Usability Dilution guideline References
ELISA Yes 1:80,000 1, 2, 3,4
Immunoblotting Yes 1:200
Immunohistochemistry Yes 1:50
Application Remarks:
 
References:

1. Underwood PA, Bennett FA (1989) A comparison of the biological activities of the cell-adhesive proteins vitronectin and fibronectin. J Cell Sci 93:641-649.
2. Underwood PA, Steele JG, Dalton BA, Bennet FA (1990). Solid phase monoclonal antibodies. A novel method of directing the function of biologically active molecules be presenting a specific concentration. J Immunol Methods 127:91-102.
3. Underwood PA, Bean PA, Mitchell SM, Whitelock JM (2001) Specific affinity depletion of cell adhesion molecules and growth factors from serum. J Immunol Methods 247:217-224.

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