Anti-Thrombospondin 1 (bovine,human)
Mouse monoclonal antibody
Cat.No. CSI 002-65
Preparation: Protein-A purified
Content: Available in 200 µL and 1 mL volumes, 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
Thrombospondin 1 (TSP1) is a multidomain, multifunctional glycoprotein from platelets and certain vascular cells which has been shown to play an important role in cell-cell and cell-matrix interactions. TSP1 is a calcium-sensitive, disulfide-bonded trimer with a subunit molecular mass of 150 kDa. TSP1 exists in different conformational states depending on the calcium ion concentration used in the purification buffers (1)
Extracellular matrix material from cultured bovine corneal endothelial cells.
CSI 002-65 is highly specific for the low-calcium (0.1 mmol/L) conformation of human TSP1. There is no evidence of cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin).
Cross-reactivity with thrombospondins from other species has not been tested.
Epitope is only present in thrombospondin prepared in low-calcium (0.1 mmol/L) buffers.
CSI 002-65 can be used for immunoprecipitation and for immunostaining of frozen periodate-lysine-paraformaldehyde-fixed sections of bovine and human tissues. CSI 002-65 can also be used to probe for the low-calcium conformation of thrombospondin.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1/200 | 1 |
| Immunoblotting | Not determined | ||
| Immunohistochemistry | Yes | 1/200 | 1 |
Users should determine the optimal dilutions for their own purposes.
1. Matthias LJ, Gotis-Graham I, Underwood PA, McNeil HP, Hogg PJ (1996) Identification of monoclonal antibodies that recognize different disulphide bonded forms of thrombospondin 1. Biochem Biophys Acta 1216:138-144.
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CSI 002-65.pdf (19.79 kB) Product specification
Cell biology flyer.pdf (197.81 kB)
Cell biology flyer
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April 23 2008
